DESCRIPTION

• Mw: 110.4 kDa
• Tag: Myc-DDK
• Accn: NM_001184793
• Type: Human Tagged ORF Clone
• Refseq: NM_001184793.1, NP_001171722.1
• Status: 5 Weeks*
• Symbol: PARD3
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 56288
• Orf Size: 3000 bp
• Synonyms: ASIP; Baz; PAR3; PAR3alpha; PARD-3; PARD3A; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2
• Refseq Orf: 3003 bp
• Uniprot Id: Q8TEW0
• Availability: 5 Weeks
• Cytogenetics: 10p11.22-p11.21
• Gene Summary: This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC230609 representing NM_001184793 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAAAGTGACCGTGTGCTTCGGACGGACCCGGGTGGTCGTGCCGTGCGGGGACGGCCACATGAAAGTTT TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCATCGCCAAGGATCCAAACTACTGGATACA GGTGCATCGCTTGGAACATGGAGATGGAGGAATACTAGACCTTGATGACATTCTTTGTGATGTAGCAGAC GATAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCACATCACGGAGGTGATGGCACCAGTGCCA GTTCCACGGGTACCCAGAGCCCAGAGATATTTGGTAGTGAGCTTGGCACCAACAATGTCTCAGCCTTTCA GCCTTACCAAGCAACAAGTGAAATTGAGGTCACACCTTCAGTCCTTCGAGCAAATATGCCTCTTCATGTT CGACGCAGTAGTGACCCAGCTCTAATTGGCCTCTCCACTTCTGTCAGTGATAGTAATTTTTCCTCTGAAG AGCCTTCAAGGAAAAATCCCACACGCTGGTCAACAACAGCTGGCTTCCTCAAGCAGAACACTGCTGGGAG TCCTAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTCCCGCGGGATACTAGTAACTGGTCT AACCAATTTCAGAGAGACAATGCTCGCTCGTCTCTGAGTGCCAGTCACCCAATGGTGGGCAAGTGGCTGG AGAAACAAGAACAGGATGAGGATGGGACAGAAGAGGATAACAGTCGTGTTGAACCTGTTGGACATGCTGA CACGGGTTTGGAGCATATACCCAACTTTTCTCTGGATGATATGGTAAAGCTCGTAGAAGTCCCCAACGAT GGAGGGCCTCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACCCTGGGGTTATTAGTAA AACGATTGGAGAAAGGTGGTAAAGCTGAACATGAAAATCTTTTTCGTGAGAATGATTGCATTGTCAGGAT TAATGATGGCGACCTTCGAAATAGAAGATTTGAACAAGCACAACATATGTTTCGCCAAGCCATGCGTACA CCCATCATTTGGTTCCATGTGGTTCCTGCAGCAAATAAAGAGCAGTATGAACAACTATCCCAAAGTGAGA AGAACAATTACTATTCAAGCCGTTTTAGCCCTGACAGCCAGTATATTGACAACAGGAGTGTGAACAGTGC AGGGCTTCACACGGTGCAGAGAGCACCCCGACTGAACCACCCGCCTGAGCAGATAGACTCTCACTCAAGA CTACCTCATAGCGCACACCCCTCGGGAAAACCACCATCCGCTCCAGCCTCGGCACCTCAGAATGTATTTA GTACGACTGTAAGCAGTGGTTATAACACCAAAAAAATAGGCAAGAGGCTTAATATCCAGCTTAAGAAAGG TACAGAAGGTTTGGGATTCAGCATCACTTCCAGAGATGTAACAATAGGTGGCTCAGCTCCAATCTATGTG AAAAACATTCTCCCCCGGGGGGCGGCCATTCAGGATGGCCGACTTAAGGCAGGAGACAGACTTATAGAGG TAAATGGAGTAGATTTAGTGGGCAAATCCCAAGAGGAAGTTGTTTCGCTGTTGAGAAGCACCAAGATGGA AGGAACTGTGAGCCTTCTGGTCTTTCGCCAGGAAGACGCCTTCCACCCAAGGGAACTGAATGCAGAGCCA AGCCAGATGCAGATTCCAAAAGAAACGAAAGCAGAAGATGAGGATATTGTTCTTACACCTGATGGCACCA GGGAATTTCTGACATTTGAAGTCCCACTTAATGATTCAGGATCTGCAGGCCTTGGTGTCAGTGTCAAAGG TAACCGGTCAAAAGAGAACCACGCAGATTTGGGAATCTTTGTCAAGTCCATTATTAATGGAGGAGCAGCA TCTAAAGATGGAAGGCTTCGGGTGAATGATCAACTGATAGCAGTAAATGGAGAATCCCTGTTGGGCAAGA CAAACCAAGATGCCATGGAAACCCTAAGAAGGTCTATGTCTACTGAAGGCAATAAACGAGGAATGATCCA GCTTATTGTTGCAAGGAGAATAAGCAAGTGCAATGAGCTGAAGTCACCTGGGAGCCCCCCTGGACCTGAG CTGCCCATTGAAACAGCGTTGGATGATAGAGAACGAAGAATTTCCCATTCCCTCTACAGTGGGATTGAGG GGCTTGATGAATCGCCCAGCAGAAATGCTGCCCTCAGTAGGATAATGGGTAAATACCAGCTGTCCCCTAC AGTGAATATGCCCCAAGATGACACTGTCATTATAGAAGATGACAGGTTGCCAGTGCTTCCTCCACATCTC TCTGACCAGTCCTCTTCCAGCTCCCATGATGATGTGGGGTTTGTGACGGCAGATGCTGGTACTTGGGCCA AGGCTGCAATCAGTGATTCAGCCGACTGCTCTTTGAGTCCAGATGTTGATCCAGTTCTTGCTTTTCAACG AGAAGGATTTGGACGTCAGACTGACGAGACTAAACTCAATACAGTGGATGACCAGAAAGCAGGTTCTCCC AGCAGAGATGTGGGTCCTTCCCTGGGTCTGAAGAAGTCAAGCTCGTTGGAGAGTCTGCAGACCGCAGTTG CCGAGGTGACTTTGAATGGGGATATTCCTTTCCATCGTCCACGGCCGCGGATAATCAGAGGCAGGGGATG CAATGAGAGCTTCAGAGCTGCCATCGACAAATCTTATGATAAACCCGCGGTAGATGATGATGATGAAGGC ATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAGATCAGGGAGAGAGTCTGTATCCACAGCCAGTG ATCAGCCTTCCCACTCTCTGGAGAGACAAATGAATGGAAACCAAGAGAAAGGTGATAAGACTGATAGAAA AAAGGATAAAACTGGAAAAGAAAAGAAGAAAGATAGAGATAAGGAGAAGGATAAAATGAAAGCCAAGAAG GGAATGCTGAAGGGCTTGGGAGACATGTTCAGCCTTGCCAAACTGAAGCCCGAGAAGAGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC230609 representing NM_001184793 Red =Cloning site Green =Tags(s) MKVTVCFGRTRVVVPCGDGHMKVFSLIQQAVTRYRKAIAKDPNYWIQVHRLEHGDGGILDLDDILCDVAD DKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHV RRSSDPALIGLSTSVSDSNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDTSNWS NQFQRDNARSSLSASHPMVGKWLEKQEQDEDGTEEDNSRVEPVGHADTGLEHIPNFSLDDMVKLVEVPND GGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEHENLFRENDCIVRINDGDLRNRRFEQAQHMFRQAMRT PIIWFHVVPAANKEQYEQLSQSEKNNYYSSRFSPDSQYIDNRSVNSAGLHTVQRAPRLNHPPEQIDSHSR LPHSAHPSGKPPSAPASAPQNVFSTTVSSGYNTKKIGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYV KNILPRGAAIQDGRLKAGDRLIEVNGVDLVGKSQEEVVSLLRSTKMEGTVSLLVFRQEDAFHPRELNAEP SQMQIPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAA SKDGRLRVNDQLIAVNGESLLGKTNQDAMETLRRSMSTEGNKRGMIQLIVARRISKCNELKSPGSPPGPE LPIETALDDRERRISHSLYSGIEGLDESPSRNAALSRIMGKYQLSPTVNMPQDDTVIIEDDRLPVLPPHL SDQSSSSSHDDVGFVTADAGTWAKAAISDSADCSLSPDVDPVLAFQREGFGRQTDETKLNTVDDQKAGSP SRDVGPSLGLKKSSSLESLQTAVAEVTLNGDIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPAVDDDDEG METLEEDTEESSRSGRESVSTASDQPSHSLERQMNGNQEKGDKTDRKKDKTGKEKKKDRDKEKDKMKAKK GMLKGLGDMFSLAKLKPEKR TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction, Tight junction
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin