Monoclonal Antibody to VEGF (Vascular Endothelial Growth Factor)(Clone : VEGF/1063)

DESCRIPTION

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

DETAILS

Gene: VEGFA
App Id: IHC
Antigen: VEGF (Vascular Endothelial Growth Factor)
Gene Id: 7422
Isotype: Mouse IgG1, kappa
Format Id: Purified
Clone Name: VEGF/1063
Uniprot Id: P15692
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Mouse, Rat
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: VEGFA||VEGF
Application Note: Immunohistochemistry (Formalin-fixed) (4-8ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Human VEGF189 recombinant protein