DESCRIPTION

• Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
• Host: Rabbit
• Clone: NA
• Label: Unconjugated
• Purity: Affinity purified on immobilized antigen.
• Target: Sodium/hydrogen exchanger 6, NHE6, SLC9A6
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 10479
• Isotype: Rabbit IgG
• Homology: Mouse, rat - 13/14 amino acid residues identical
• Is Toxin: No
• Sequence: (C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6
• Synonyms: Sodium/hydrogen exchanger 6, NHE6, SLC9A6
• Accession: Q92581
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Rat|Mouse
• Formulation: PBS pH7.4
• Applications: IF|IHC|WB
• Preservative: No Preservative
• Reconstitution: 0.2 ml double distilled water (DDW).
• Blocking Peptide: BLP-NX006
• Negative Control: BLP-NX006
• Positive Control: NA
• Immunogen Location: Intracellular, C-terminus
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Human
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IF|IHC|WB
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: Intracellular pH regulation in the brain is extremely important in both physiological and pathophysiological conditions. Changes in Intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located on the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1,2. In addition to their role in regulating pH, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1.Several studies have suggested that Na+/H+ exchange is the predominant pH-regulatory mode in isolated nerve terminals. In addition, Na+/H+ exchange plays a role in regulating synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses1.The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane, NHE1-9 encoded by the SLC9A1-SLC9A9 genes, respectively. SLC9 family members have a short N terminus and an extensive C terminus that plays a regulatory role2.NHE6 is localized to early and recycling endosomes, where it exchanges luminal H+ for Na+ or K+. Its expression is highest in the spinal cord and brain and is particularly enriched in the hippocampus, cortex, and Purkinje cell layer of the cerebellum3. Mutations in NHE6 encoding gene have been linked to autism, Christianson syndrome, and Angelman-like syndrome.
• Standard Quality Control of Each Lot: Western blot analysis
• Application Dilutions Western Blot Wb: 1:200
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:200