The POLQ Helicase ATPase Assay System is intended for use with the Transcreener® ADP2 Assay Kits to measure the DNA-dependent ATPase activity of the POLQ Helicase domain. POLQ (also known as DNA Polymerase Theta), is a multifunctional enzyme with template-dependent DNA polymerase, ATPdependent helicase, and dNTP-dependent endonuclease activities that play important roles in double strand break repair. ADP formation by the ATPase activity of the POLQ Helicase domain can be easily measured by the Transcreener® ADP2 assay, a far-red, competitive fluorescence assay that enables single addition, mix-and-read detection in a continuous or endpoint format. The assay has been optimized and extensively validated for high throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers.