DESCRIPTION

• Target: HO-1
• Gene Id: 3162
• Conjugate: APC
• Immunogen: Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
• References: 1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
• Swiss-prot: P09601
• Specificity: Detects ~32kDa.
• Applications: WB | ICC/IF | IHC
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Rabbit
• Product Type: Polyclonal
• Purification: Protein A Purified
• Concentration: 1 mg/ml
• Research Areas: Cancer | Oxidative Stress | Neuroscience | Neurodegeneration | Alzheimer's Disease | Cardiovascular System | Blood | Platelets | Cell Signaling | Epigenetics and Nuclear Signaling | Nuclear Signaling Pathways | NFkB Pathway | Cardiovascular System | Atherosclerosis | Vascular Inflammation | Inflammatory Mediators | Cardiovascular System | Vasculature | Endothelium | Cancer | Cancer Metabolism | Response to Hypoxia | Cell Signaling | Metabolism | Metabolism processes | Hypoxia
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_002124.1
• Alternative Names: HSP32 Antibody, HMOX1 Antibody, Heme oxygenase 1 Antibody, HO Antibody, HO1 Antibody, , 32 kD antibody, bK286B10 antibody, D8Wsu38e antibody, heat shock protein 32 kD antibody, heat shock protein 32kD antibody, Heat shock protein antibody, Heme oxygenase (decycling) 1 antibody, Heme oxygenase 1 antibody, Hemox antibody, HMOX 1 antibody, Hmox antibody, Hmox1 antibody, HMOX1_HUMAN antibody, HO 1 antibody, HO antibody, HO-1 antibody, HO1 antibody, Hsp32 antibody
• Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SPC-112D-APC, RRID: AB_2703305
• Immunogen Species: Human
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Endoplasmic Reticulum | Microsome
• Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
• Certificate of Analysis: 1 µg/ml of SPC-112 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat | Dog