51099-M08H
Mouse Erythropoietin / EPO Protein (His Tag)
Sino Biological
DESCRIPTION
A DNA sequence encoding the mouse EPO (P07321) (Met1-Arg192) was expressed with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: > 96 % as determined by SDS-PAGE
- Source: HEK293 Cells
- Species: Mouse
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1. Measured by its ability to bind biotinylated human EPOR-His (Cat:10707-H08H) in a functional ELISA. 2. Measured by its ability to bind mouse EPOR-Fch (Cat:50031-M03H) in a functional ELISA. 3. Measured by its ability to bind human EPOR & CD131-Fc (Cat:CT010-H02H) in a functional ELISA. 4. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
- Molecule: EPO
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: P07321
- Predicted N Terminal: Ala 27