DESCRIPTION

• Aa: 2-186
• Mw: 23 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Purity: ≥90%
• Genbank: NM_033360
• Species: Human
• Uniprot: P01116
• Shiptemp: -80°C (dry ice)
• Synonyms: C-K-RAS, CFC2, K-ras, kRas(G12D), kRas, KRAS proto-oncogene, GTPase, Kirsten Rat Sarcoma virus, NS, NS3, OES, RALD, KRAS1, KRAS2, RASK2, KI-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, c-Ki-ras, c-Ki-ras2
• Warnings: Avoid freeze/thaw cycles.
• Category: Ras Signaling/Protein
• Constructs: KRAS (G12D) (His-TEV-2-186)-(GDP)
• Description: Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.
• Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT
• Supplied As: Aqueous buffer solution
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was performed according to the KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) with GDP loaded KRAS (G12C) (BPS Bioscience #100640) and GDP loaded KRAS (G12D) (BPS Bioscience #101312) titrated from 200 nM to 0 nM. Enzyme was incubated with SOS1; positive results show that SOS1 mediated exchange and subsequent RAS-Raf binding is successful.
• Related Products: 100640, 100641, 100623, 100887
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Cell Signaling Pathway