DESCRIPTION

• Mw: 90.8 kDa
• Tag: Tag Free
• Accn: NM_001144063
• Type: Human Untagged Clone
• Refseq: NM_001144063.1
• Status: 3 Weeks*
• Symbol: OSBPL5
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 114879
• Synonyms: OBPH1; ORP5
• Refseq Orf: 2436 bp
• Uniprot Id: Q9H0X9
• Insert Size: 2436 bp
• Refseq Size: 3700 bp
• Availability: 3 Weeks
• Cytogenetics: 11p15.4
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that play a key role in the maintenance of cholesterol balance in the body. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. This gene has been shown to be imprinted, with preferential expression from the maternal allele only in placenta. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010] Transcript Variant: This variant (3) lacks an in-frame exon compared to variant 1, resulting in a shorter isoform (b) compared to isoform a. Isoform b has a sterol-binding domain but lacks a complete pleckstrin homology domain. Variants 2 and 3 encode the same protein.
• Sequence Data: Fully Sequenced ORF ( show hide ) >SC325338 representing NM_001144063. Blue =Insert sequence Red =Cloning site Green =Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGC C ATGAAGGAGGAGGCCTTCCTCCGGCGCCGCTTCTCCCTGTGTCCACCTTCCTCCACCCCTCAGAAAGTC GACCCCCGGAAGCTCACCCGGAACTTGCTCCTCAGCGGAGACAATGAGCTCTACCCACTCAGCCCAGGG AAGGACATGGAGCCCAACGGCCCGTCGCTGCCCAGGGATGAAGGGCCCCCGACCCCAAGCTCTGCCACG AAGGTGCCACCGGCAGAGTACAGGCTGTGCAACGGGTCAGACAAGGAATGTGTGTCCCCCACCGCCAGG GTCACCAAGAAGGAGACTCTCAAGGCGCAGAAGGAGAACTACCGGCAGGAGAAGAAGCGCGCCACACGG CAGCTGCTCAGCGCTCTGACAGACCCCAGCGTGGTCATCATGGCTGACAGCCTGAAGGGCCCCAAAGGT GAGAGCGTGGGCTCCATCACACAGCCCCTGCCCAGCAGCTACCTGATCTTCAGGGCCGCCTCCGAGTCA GATGGTCGCTGCTGGCTGGACGCCCTGGAGCTGGCCCTGCGCTGCTCTAGCCTACTGAGACTGGGCACC TGCAAGCCGGGCCGAGACGGGGAGCCAGGGACCTCGCCAGACGCATCACCCTCATCGCTCTGTGGGCTG CCAGCCTCAGCCACCGTCCACCCAGACCAAGACCTGTTCCCACTGAACGGGTCTTCCCTGGAGAACGAT GCATTCTCAGACAAGTCGGAGAGAGAGAACCCTGAGGAGTCAGATACCGAGACCCAGGACCATAGCCGG AAGACGGAGAGTGGCAGCGACCAGTCAGAGACCCCTGGGGCCCCGGTGCGGAGAGGGACCACCTATGTG GAGCAGGTCCAGGAGGAGCTGGGGGAGCTGGGCGAGGCGTCCCAGGTGGAGACAGTGTCAGAGGAGAAC AAGAGTCTGATGTGGACCCTGCTGAAGCAGCTACGGCCAGGCATGGACCTGTCCCGCGTGGTGCTACCC ACGTTCGTACTGGAGCCGCGCTCCTTCCTGAACAAGCTCTCCGACTACTACTACCACGCAGACCTGCTC TCCAGGGCTGCGGTGGAGGAGGATGCCTACAGCCGCATGAAGCTGGTGCTGCGGTGGTACCTGTCTGGC TTCTACAAGAAGCCCAAGGGAATCAAGAAGCCGTACAACCCCATCCTGGGGGAGACCTTCCGCTGCTGC TGGTTCCACCCGCAGACTGACAGCCGCACATTCTACATAGCAGAGCAGGTGTCCCACCACCCGCCCGTG TCTGCCTTCCACGTCAGCAACCGGAAGGACGGCTTCTGCATCAGTGGCAGCATCACAGCCAAGTCCAGG TTTTATGGGAACTCGCTGTCGGCGCTGCTGGACGGCAAAGCCACGCTCACCTTCCTGAACCGAGCCGAG GATTACACCCTTACCATGCCCTACGCCCACTGCAAAGGAATCCTGTATGGCACGATGACCCTGGAGCTG GGTGGGAAGGTCACCATCGAGTGTGCGAAGAACAACTTCCAGGCCCAGCTGGAATTCAAACTCAAGCCC TTCTTCGGGGGTAGCACCAGCATCAACCAGATCTCGGGAAAGATCACGTCGGGAGAGGAAGTCCTGGCG AGCCTCAGTGGCCACTGGGACAGGGACGTGTTTATCAAGGAGGAAGGGAGCGGAAGCAGTGCGCTTTTC TGGACCCCGAGCGGGGAGGTCCGCAGACAGAGGCTGAGGCAGCACACGGTGCCGCTGGAGGAGCAGACG GAGCTGGAGTCCGAGAGGCTCTGGCAGCACGTCACCAGGGCCATCAGCAAGGGCGACCAGCACAGGGCC ACACAGGAGAAGTTTGCACTGGAGGAGGCACAGCGGCAGCGGGCCCGTGAGCGGCAGGAGAGCCTCATG CCCTGGAAGCCGCAGCTGTTCCACCTGGACCCCATCACCCAGGAGTGGCACTACCGATACGAGGACCAC AGCCCCTGGGACCCCCTGAAGGACATCGCCCAGTTTGAGCAAGACGGGATCCTGCGGACCTTGCAGCAG GAGGCCGTGGCCCGCCAGACCACCTTCCTGGGCAGCCCAGGGCCCAGGCACGAGAGGTCTGGCCCAGAC CAGCGGCTTCGCAAGGCCAGCGACCAGCCCTCCGGCCACAGCCAGGCCACGGAGAGCAGCGGATCCACG CCTGAGTCCTGCCCAGAGCTCTCAGACGAGGAGCAGGATGGTGACTTTGTCCCTGGCGGTGAGAGCCCA TGCCCTCGGTGCAGGAAGGAGGCGCGGCGGCTGCAGGCCCTGCACGAGGCCATCCTCTCCATCCGAGAG GCCCAGCAGGAGCTGCACAGGCACCTCTCGGCCATGCTGAGCTCCACGGCACGGGCAGCACAGGCACCG ACCCCAGGCCTCCTGCAGAGCCCCCGATCCTGGTTCCTGCTCTGCGTGTTCCTGGCGTGTCAGCTGTTC ATTAACCACATCCTCAAATAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAG GTTTAAACGGCCGGC
• Oti Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin