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AOR-015-AR_50 mcl

Scientist.com Supplier

Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody

Alomone Labs

DESCRIPTION

Anti-Nociceptin Receptor (OPRL1) Antibody (#AOR-015) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot analysis, and has been designed to recognize the nociceptin receptor from mouse, rat and human samples. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody (#AOR-015-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

DETAILS

  • Form: Lyophilized powder
  • Host: Rabbit
  • Clone: NA
  • Label: ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
  • Purity: Affinity purified on immobilized antigen.
  • Target: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 29256
  • Isotype: Rabbit IgG
  • Homology: Mouse - identical; human - 13/16 amino acid residues identical
  • Is Toxin: No
  • Sequence: (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1
  • Synonyms: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor
  • Accession: P35370
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
  • Applications: IF|IHC
  • Preservative: 1% BSA, 0.05% NaN3
  • Reconstitution: 50 µl double distilled water (DDW).
  • Blocking Peptide: BLP-OR015
  • Negative Control: BLP-OR015
  • Positive Control: NA
  • Immunogen Location: Intracellular, C-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Rat
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IF|IHC
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: Nociceptin/orphanin FQ (N/OFQ), a 17 amino acid peptide, is the endogenous agonist of the nociceptin/orphanin FQ peptide (NOP) receptor (previously termed ORL1 receptor) which belongs to the class A (Rhodopsin-like) γ subfamily of G protein-coupled receptors (GPCRs). Like all members the receptor has a common seven-transmembrane helical structure and is coupled predominantly to heterotrimeric Gi/Go proteins. N/OFQ receptor shows a high degree of structural homology with the μ-, δ- and κ-opioid receptors1. The intracellular events triggered by the binding of N/OFQ with the NOP receptor include inhibition of adenylyl cyclase, activation of phospholipase C (PLC) and of K+ channels, inhibition of Ca2+ channels, and activation of mitogen-activated protein-kinases (MAPKs). In neural tissues, the main effect is the inhibition of the release of neurotransmitters (acetylcholine, catecholamines, GABA, glutamate, 5-hydroxytryptamine, tachykinines)2.NOP receptor mRNA is detected in high levels in the cortex, anterior olfactory nucleus, lateral septum, hypothalamus, hippocampus, amygdala, central grey, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus and spinal cord3.Studies with N/OFQ, NOP-selective agonists or antagonists, and receptor- or peptide-deficient mice have shown that the NOP system has important roles in the control of central and peripheral functions including pain, anxiety depression, hyperphagia and obesity, addiction, Parkinson's disease, cognition, food intake, learning and memory, locomotion, cough and micturition reflexes, cardiovascular homeostasis, intestinal motility and immune responses4.
  • Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #AOR-015), and immunohistochemistry (labeled antibody).
  • Application Dilutions Western Blot Wb: Contact Alomone for information
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:60