Sirtuin 6 Fluorogenic Assay Kit

DESCRIPTION

The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.

DETAILS

Uniprot: Q8N6T7
Shiptemp: -80°C (dry ice)
Synonyms: SIRT6, Sirtuin 6, SIRT, SIRT 6, Fluorogenic Sirtuin 6 Assay Kit
Warnings: Avoid freeze/thaw cycles.
Category: Deacetylase/Assay Kit
Background: Sirtuins are NAD+ dependent class III histone deacetylases that regulate important biological processes including metabolism and aging. In human, there are seven isoforms of Sirtuins, SIRT1 to SIRT7. Four of the isoforms, SIRT4 to SIRT7, have no detectable or very weak deacetylase activity. Sirtuin 6 (SIRT6) is shown to be a potent defatty-acylase of lysine residues.
References: 1. Hu, J. et al., Org Miomol Chem, 2013. 11(32):5213-5216.
Description: The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.
Methodology: Fluorogenic
Supplied As: Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control,
Unspsc Code: 41105331
Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 50089, 50090, 50017, 50080
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Scientific Category: Deacetylase
Instructions for Use: See assay kit datasheet for detailed protocol.