p180-292t p180-292
Promo Available
Anti-MEK1 (Thr292) Antibody
PhosphoSolutions
DESCRIPTION
Our Anti-MEK1 (Thr292) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse MEK1 (Thr292) and is antigen affinity purified from pooled serum. It is great for use in WB.
DETAILS
- Form: Antigen Affinity Purified from Pooled Serum
- Gene: MAP2K1
- Host: Rabbit
- Rrid: AB_2492147
- Type: Primary Antibody
- Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
- Target: MEK1 (Thr292)
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated kinase 1 antibody
- Clonality: Polyclonal
- Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH).
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr292.
- Applications: WB
- Ncbi Gene Id: 5604
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: 45
- Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
- Dilution Range Wb: 1:1000
- Immunogen Species: Human
- Species Reactivity: Human and Mouse
- Target Description: MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr-292 and Thr-386 (Mansour et al., 1994).
- Expected Reactivity: Bovine, Canine, Non-Human Primate, Rat
- Uniprot Number Immunogen Species: Q02750
- Post Translational Modification Type: Phosphorylated
- Post Translational Modification Residue: Thr292
PROMOTION TERMS
Trial Size Available. Limit 1
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