10560-H08B
Human DOPA Decarboxylase / DDC Protein (His Tag)
Sino Biological
DESCRIPTION
A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
DETAILS
- Tag: C-His
- Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
- Source: Baculovirus-Insect Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.
- Molecule: DDC
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Supplied as sterile 50mM Tris, 100mM NaCl, pH 8, 10% glycerol
- Molecular Mass: The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_000781.1
- Predicted N Terminal: Met