DESCRIPTION

• Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
• Species: Human
• Uniprot: Q13043
• Shiptemp: -80°C (dry ice)
• Synonyms: Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1 (MST-1), STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, KRS2, MST1
• Warnings: Avoid freeze/thaw cycles.
• Category: Cancer Therapy Target
• Background: STK4 (serine/threonine-protein kinase 4), also known as MST1 (Mammalian STE20-like protein kinase 1), belongs to a family of proteins that share similarity with budding yeast serine/threonine kinase Ste20 (sterile-20). It is a core component of the Hippo signaling pathway, the pathway that regulates organ size via cell proliferation or apoptosis and is widely expressed throughout the human body. In the presence of stressful stimuli, autophosphorylation of STK4 at Thr183 initiates caspase-mediated cleavage to create a highly active, 36 kDa STK4 fragment. Examples of stressors are okadaic acid (protein phosphatase PP-1 and PP-2A inhibitor) and staurosporine (a broad-spectrum protein kinase inhibitor). Overexpression of STK4 can initiate apoptosis in mammalian cells when transiently expressed with MST2, a closely related Class II GC (protein Ser/Thr) Kinase, or in the presence of SAPK (stress-activated protein kinase). Inhibition of STK4 can lead to failure to arrest proliferation or to developmental apoptosis in certain cell types. STK4 is a potential diagnostic biomarker and therapeutic target for the treatment of various types of cancer.
• References: Praskova M, et al., Biochem J. 2004; 381(Pt 2):453-462. Li W, et al., J Clin Invest. 2015 Nov 2; 125(11):4239-4254.
• Description: The STK4(MST1) Kinase Assay Kit is designed to measure STK4(MST1) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. 
• Methodology: Chemiluminescent
• Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant STK4(MST1) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 40276, 40133, 40013, 40074, 78487
• Contraindications: The final concentration of DMSO in the assay should not exceed 1%. 
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 
• Scientific Category: Kinase