DESCRIPTION

• Mw: 97.6 kDa
• Tag: Myc-DDK
• Accn: NM_001270660
• Type: Human Tagged ORF Clone
• Refseq: NM_001270660.2
• Status: 5 Weeks*
• Symbol: MARCHF6
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 10299
• Orf Size: 2586 bp
• Synonyms: DOA10; FAME3; MARCH-VI; MARCH6; RNF176; TEB4
• Refseq Orf: 2589 bp
• Uniprot Id: O60337
• Refseq Size: 9395 bp
• Availability: 5 Weeks
• Cytogenetics: 5p15.2
• Gene Summary: This gene encodes a member of a family of membrane-associated E3 ubiquitin ligases containing RING-CH-type zinc finger motifs. Ubiquitination of type II deiodinase by the encoded protein is an important regulatory step in thyroid hormone signalling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC231503 representing NM_001270660 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGACACCGCGGAGGAAGACATATGTAGAGTGTGTCGGTCAGAAGGAACACCTGAGAAACCGCTTTATC ATCCTTGTGTATGTACTGGCAGTATTAAGTTTATCCATCAAGAATGCTTAGTTCAATGGCTGAAACACAG TCGAAAAGAATACTGTGAATTATGCAAGCACAGATTTGCTTTTACACCAAGCCGCATCTACAAGTGCTTG TTTACTGGCTCCGTGAGCTCACTACTGACGCTGCCATTAGATATGCTGTCAACGGAAAATTTGTTGGCAG ATTGTTTGCAGGGTTGTTTTGTGGTGACGTGCACACTGTGTGCATTCATCAGCCTGGTGTGGTTGAGAGA GCAGATAGTCCATGGGGGAGCACCAATTTGGTTGGAGCATGCTGCCCCACCGTTCAATGCTGCGGGGCAT CACCAAAATGAGGCTCCAGCAGGAGGAAATGGTGCAGAAAATGTTGCTGCTGATCAGCCTGCTAACCCAC CAGCTGAGAACGCAGTGGTGGGGGAAAACCCTGATGCCCAGGATGACCAGGCAGAAGAGGAGGAGGAGGA CAATGAGGAGGAAGATGACGCTGGTGTGGAGGATGCGGCAGATGCTAATAACGGAGCCCAGGATGACATG AATTGGAATGCTTTAGAATGGGACCGAGCTGCTGAAGAGCTTACATGGGAAAGAATGCTAGGACTTGATG GATCACTAGTTTTTCTGGAACATGTCTTCTGGGTGGTATCTTTAAATACACTGTTCATTCTTGTTTTTGC ATTTTGCCCTTACCATATTGGTCATTTCTCCCTTGTTGGTTTGGGATTTGAAGAACACGTCCAAGCATCT CATTTTGAAGGCCTAATCACAACCATAGTTGGGTATATACTTTTAGCAATAACACTGATAATTTGTCATG GCTTGGCAACTCTTGTGAAATTTCATAGATCTCGTCGCTTACTGGGAGTCTGCTATATTGTTGTTAAGGT CTCTTTGTTAGTGGTGGTAGAAATTGGAGTATTCCCTCTCATTTGTGGTTGGTGGCTGGATATCTGTTCC TTGGAAATGTTTGATGCTACTCTGAAAGATCGAGAACTGAGCTTTCAGTCGGCTCCAGGTACTACCATGT TTCTGCATTGGCTAGTGGGAATGGTATATGTCTTCTACTTTGCCTCCTTCATTCTACTACTGAGAGAGGT ACTTCGACCTGGTGTCCTGTGGTTTCTAAGGAATTTGAATGATCCAGATTTCAATCCAGTACAGGAAATG ATCCATTTGCCAATATATAGGCATCTCCGAAGATTTATTTTGTCAGTGATTGTCTTTGGCTCCATTGTCC TCCTGATGCTTTGGCTTCCTATACGTATAATTAAGAGTGTGCTGCCTAATTTTCTTCCATACAATGTCAT GCTCTACAGTGATGCTCCAGTGAGTGAACTGTCCCTCGAGCTGCTTCTGCTTCAGGTTGTCTTGCCAGCA TTACTCGAACAGGGACACACGAGGCAGTGGCTGAAGGGGCTGGTGCGAGCGTGGACTGTGACCGCCGGAT ACTTGCTGGATCTTCATTCTTATTTATTGGGAGACCAGGAAGAAAATGAAAACAGTGCAAATCAACAAGT TAACAATAATCAGCATGCTCGAAATAACAACGCTATTCCTGTGGTGGGAGAAGGCCTTCATGCAGCCCAC CAAGCCATACTCCAGCAGGGAGGGCCTGTTGGCTTTCAGCCTTACCGCCGACCTTTAAATTTTCCACTCA GGATATTTCTGTTGATTGTCTTCATGTGTATAACATTACTGATTGCCAGCCTCATCTGCCTTACTTTACC AGTATTTGCTGGCCGTTGGTTAATGTCGTTTTGGACGGGGACTGCCAAAATCCATGAGCTCTACACAGCT GCTTGTGGTCTCTATGTTTGCTGGCTAACCATAAGGGCTGTGACGGTGATGGTGGCATGGATGCCTCAGG GACGCAGAGTGATCTTCCAGAAGGTTAAAGAGTGGTCTCTCATGATCATGAAGACTTTGATAGTTGCGGT GCTGTTGGCTGGAGTTGTCCCTCTCCTTCTGGGGCTCCTGTTTGAGCTGGTCATTGTGGCTCCCCTGAGG GTTCCCTTGGATCAGACTCCTCTTTTTTATCCATGGCAGGACTGGGCACTTGGAGTCCTGCATGCCAAAA TCATTGCAGCTATAACATTGATGGGTCCTCAGTGGTGGTTGAAAACTGTAATTGAACAGGTTTACGCAAA TGGCATCCGGAACATTGACCTTCACTATATTGTTCGTAAACTGGCAGCTCCCGTGATCTCTGTGCTGTTG CTTTCCCTGTGTGTACCTTATGTCATAGCTTCTGGTGTTGTTCCTTTACTAGGTGTTACTGCGGAAATGC AAAACTTAGTCCATCGGCGGATTTATCCATTTTTACTGATGGTCGTGGTATTGATGGCAATTTTGTCCTT CCAAGTCCGCCAGTTTAAGCGCCTTTATGAACATATTAAAAATGACAAGTACCTTGTGGGTCAACGACTC GTGAACTACGAACGGAAATCTGGCAAACAAGGCTCATCTCCACCACCTCCACAGTCATCCCAAGAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC231503 representing NM_001270660 Red =Cloning site Green =Tags(s) MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPSRIYKCL FTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGH HQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDM NWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQAS HFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICS LEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEM IHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPA LLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAH QAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTA ACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLR VPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLL LSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRL VNYERKSGKQGSSPPPPQSSQE TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Transmembrane
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin