DESCRIPTION

• Category: ZYMO KITS
• Keywords: R2072, directzol, directsol, direct zol, acid-guanidinium-phenol, direct RNA prep, direct RNA purification, RNA purification
• Paragraph 1: The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 µg per prep (depending on kit selected) of high-quality RNA directly from samples in TRI Reagent® or similar.  Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.)
• Paragraph 2: Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
• Bullet Point 1: Quick, spin column purification of high-quality total RNA (17 nt or greater) directly from TRI-Reagent®, TRIzol®, or similar.
• Bullet Point 2: Bypasses messy phase separation and precipitation procedures.
• Bullet Point 3: Ideal for cells, tissues, biological liquids, in vitro transcripts, etc.
• Bullet Point 4: Efficient and consistent broad range purification of small and large RNAs.
• Bullet Point 5: High quality RNA (A260/A280 <1.8, A260/A230 <1.8) is recovered.
• Bullet Point 6: Processing Time: 10 min
• Bullet Point 8: TRI Reagent® inhibits RNase activity and inactivates viruses and other infectious agents.
• Manufacturer Sku: R2072