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CDG-C8219-25ug CDG-C8219-200ug

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

ACROBiosystems

DESCRIPTION

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-C8219) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)).

DETAILS

  • Tag: C-10×His & C-Avi | C-Flag
  • Host: HEK293
  • Size: 25ug
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: MABSol® Biotin Labeled Proteins
  • Molecule: CD3E & CD3G
  • Accession: NP_001270544.1 & NP_001270839.1
  • Conjugate: Biotin-labeled
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Exp Region: Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)
  • Swiss-prot: Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)
  • Characteristics: Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 17.7 kDa (CD3E) and 15.4 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-20 kDa and 22-24 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 17.7 kDa (CD3E) & 15.4 kDa (CD3G)
  • Shipping Condition: RT
  • Species Reactivity: Cynomolgus
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html