Plant Protein Extraction Kit: The Plant Protein Extraction Kit provides rapid recovery (as few as 20 minutes) of soluble proteins from plant tissue samples. The final protein extract is compatible with many downstream applications, including SDS-PAGE, 2-D gel electrophoresis, Western blotting, activity assays and affinity purification. The Kit includes three components: an organic solution to lyse plant cells, an aqueous buffer to gently solubilize proteins and a protein-stabilizing reagent. The Kit has been tested on leaves, roots, flowers and seeds from various species, including Arabidopsis, tobacco, spinach, peas and soybeans. Extremely fibrous tissue samples, such as woody stems, may require additional mechanical grinding by devices not included in the kit. Depending on the particular application, additional components, such as phosphatase inhibitor and chelating agents may be added to thebuffer. The kit is sufficient to extract protein from either 20 × 40 mg of fresh/frozen plant tissue or 20× 10 mg of dried plant tissue.