79741
UBCH13 TR-FRET Assay Kit
BPS Bioscience
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DESCRIPTION
UBCH13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze the formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBCH13 is required for signaling by a variety of receptors important in immune regulation, making it a candidate target for inflammatory diseases. UBCH13 is also critical for double-strand DNA repair and thus a potential radiosensitizer and chemosensitizer target for oncology.
The UBCH13 TR-FRET Assay Kit is designed to measure UBCH13 ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and a terbium-labeled anti-His antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
DETAILS
- Shiptemp: -80°C (dry ice)
- Synonyms: UBE2N, HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, ubiquitin conjugating enzyme E2 N
- Warnings: Avoid multiple freeze/thaw cycles.
- Category: Ubiquitination/Assay Kit
- References: Madiraju, C., et al., J. Biomol. Screen. 2012;17(2):163-76.
- Description: UBCH13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze the formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBCH13 is required for signaling by a variety of receptors important in immune regulation, making it a candidate target for inflammatory diseases. UBCH13 is also critical for double-strand DNA repair and thus a potential radiosensitizer and chemosensitizer target for oncology. The UBCH13 TR-FRET Assay Kit is designed to measure UBCH13 ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and a terbium-labeled anti-His antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
- Methodology: TR-FRET
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 80315, 80323, 79593, 11236
- Storage Stability: At least 6 months from date of receipt when stored as directed.
- Scientific Category: Ubiquitin
- Instructions for Use: See assay protocol for detailed instructions.