DESCRIPTION

• Aa: 656-1122 (end)
• Mw: 51 kDa
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_005474
• Species: Human
• Uniprot: Q9UQL6
• Shiptemp: -80°C (dry ice)
• Synonyms: histone deacetylase 5, HDAC-5
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Enzymes
• References: 1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-417762. Waltregny et al., (2004) J. Neural Transm. 48, 273-290. Application References: 1. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014) 2. Identification and characterization of small-molecule inhibitors of lysine acetyltransferases (2014) 3. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014) 4. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014) 5. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013) 6. Fass, D.M., et al. Neuropharmacology. 2013 Jan; 64: 81–96. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013) 7. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
• Description: Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme
• Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM BPS HDAC class 2a substrate , BPS Catalog number 50040, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
• Specific Activity: ≥1000 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Deacetylase