52088
Histone Octamer H3K27C Unmodified, His-tag Recombinant
BPS Bioscience
DESCRIPTION
Human recombinant histone tetramer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.
DETAILS
- Mw: 116 kDa
- Tags: N-terminal His-tag
- Format: Aqueous buffer solution
- Genbank: NM_033445, NM_003528, NM_003532, and NM_003548
- Species: Human
- Uniprot: P68431
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles.
- Category: Methyltransferase/Substrates
- Background: The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification
- References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012. 2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46
- Description: Human recombinant histone tetramer consisting of 2 molecules each of histones H2A (a.a. 1-130(end)), H2B (a.a. 1-126(end)), H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Global MW = 116 kDa.
- Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: A perfect substrate for histone methyltransferases.
- Product Type: Protein
- Host Cell Line: E. coli
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 52037, 52090, 52091
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Methyltransferase