DESCRIPTION

• Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
• Shiptemp: -80°C (dry ice)
• Synonyms: KIAA1240, AAA domain containing 2B (variant 1)
• Warnings: Avoid freeze/thaw cycles.
• Category: Bromodomain/Assay Kit
• References: Filippakopoulos, P., et al., Cell 2012; 149:214.
• Description: The ATAD2A TR-FRET Assay Kit is designed to measure the inhibition of ATAD2A binding to its substrate in a homogeneous 384 reaction format. This FRETbased assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, ATAD2A, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
• Methodology: TR-FRET
• Supplied As: Kit comes in a convenient format with purified ATAD2A, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 32601, 31121
• Storage Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Bromodomain
• Instructions for Use: See assay kit datasheet for detailed protocol.