CDK12 (696-1082)/CyclinK, GST-Tags, Recombinant

DESCRIPTION

Recombinant human Complex of CDK19 and Cyclin. Recombinant human CDK1 (cyclin dependent kinase 12), encompassing amino acids 696-1082. This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. Recombinant human Cyclin K (CCNK), full length, encompassing amino acids 2-580(end). This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. These two recombinant proteins were co-expressed and affinity purified.

DETAILS

Aa: CDK12: (696-1082); CyclinK: 2-580(end)
Mw: CDK12: 72 kDa; Cyclin K: 90 kDa
Tags: N-terminal GST-tag
Format: Aqueous buffer solution
Purity: ≥90%
Genbank: CDK12: NM_016507; Cyclin K: NM_001099402
Species: Human
Uniprot: CDK12: Q9NYV4; Cyclin K: O75909
Shiptemp: -80°C (dry ice)
Synonyms: Cyclin-dependent kinase 12, Cdc2-related kinase, arginine/serine-rich, CrkRS, Cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, Cell division protein kinase 12, hCDK12, CRK7, KIAA0904, Cyclin-K, CCNK, CPR4
Warnings: Avoid freeze/thaw cycles.
Category: Kinase/Protein
Description: Recombinant human Complex of CDK19 and Cyclin. Recombinant human CDK1 (cyclin dependent kinase 12), encompassing amino acids 696-1082. This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. Recombinant human Cyclin K (CCNK), full length, encompassing amino acids 2-580(end). This construct contains an N-terminal GST-tag and includes a Thrombin Cleavage Site to facilitate removal of the GST portion. These two recombinant proteins were co-expressed and affinity purified.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Host Species: insect
Product Type: Protein
Host Cell Line: Sf9 cells
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: Assay was run according to CDK12/Cyclin K Kinase Assay Kit (BPS Bioscience #78298) in a Kinase buffer containing 2 mM DTT using 0.4 mg/ml CDK12/CyclinK substrate as a substrate with 10 µM ATP at 30°C for 90 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent (Promega).
Related Products: 40106, 101128, 78298, 101235, 78487
Specific Activity: 8 pmol/min/µg
Storage Stability: At least 6 months at -80°C.
Scientific Category: Kinase