SEKA10170
Human DKK1 / Dkk-1 ELISA Pair Set
Sino Biological
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DESCRIPTION
The Human DKK1 / Dkk-1 ELISA Pair Set is for the quantitative determination of Human DKK1 / Dkk-1.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human DKK1 / Dkk-1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human DKK1 / Dkk-1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human DKK1 / Dkk-1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human DKK1 / Dkk-1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 20 ng of recombinant Human DKK1 / Dkk-1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 39.06-2500
- Specificity: Human DKK1 / Dkk-1
- Capture Antibody: 0.5 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10170-MM08)
- Detection Antibody: 0.2 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10170-MM03)