DESCRIPTION

• Aa: 1-531 (end)
• Mw: 61 kDa
• Tags:  C-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_00113486
• Species: Rat
• Shiptemp: -80°C (dry ice)
• Synonyms: ceramide kinase
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Lipid Kinase
• References: 1. Sugiura, M. et al., JBC. 277, 23294-23300 (2002) 2. Majjalieh, S.M., et al., JBC. 264, 14354-14360 (1989)
• Description: Rat CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: CERK activity was measured at 37°C for 25 min in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).
• Related Products: 40619, 40618, 40617, 79413, 78487
• Specific Activity: ≥20 pmole/min/μg
• Storage Stability: At least 6 months at –80°C. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
• Scientific Category: Kinase