AGT-005-AR_50 mcl
Scientist.com Supplier
Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
Alomone Labs
DESCRIPTION
Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize VGAT from human, mouse, and rat samples. Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody has been tested in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.
DETAILS
- Form: Lyophilized powder
- Host: Rabbit
- Clone: NA
- Label: ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
- Purity: Affinity purified on immobilized antigen.
- Target: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 83612
- Isotype: Rabbit IgG
- Homology: Mouse, human - identical
- Is Toxin: No
- Sequence: (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter
- Synonyms: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1
- Accession: O35458
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
- Applications: IF|IHC
- Preservative: 1% BSA, 0.05% NaN3
- Reconstitution: 50 µl double distilled water (DDW).
- Blocking Peptide: BLP-GT005
- Negative Control: BLP-GT005
- Positive Control: NA
- Immunogen Location: Cytoplasmic, N-terminus
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Rat
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: IF|IHC
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page - Scientific Background: The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.VGAT is expressed on synaptic vesicles of GABAergic and glycinergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.
- Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #AGT-005), and immunohistochemistry (labeled antibody).
- Application Dilutions Western Blot Wb: Contact Alomone for information
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:100