Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: MYH9
Host: Rabbit
Rrid: AB_1944447
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Myosin IIA Heavy Chain (C-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: NMHC-IIA, MYH9, myosin heavy chain
Clonality: Rabbit Polyclonal
Immunogen: NMHC-IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 200 kDa* protein corresponding to the apparent molecular mass of NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 200
Dilution Range Wb: 1:2000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human, Mouse, and Rat
Target Description: Nonmuscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.
Uniprot Number Immunogen Species: P35579