Anti-Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Recombinant Rabbit Monoclonal Antibody (Clone:BCL2/1878R)

DESCRIPTION

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2?lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2?lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

DETAILS

Gene: BCL2
App Id: FACS,IF,WB,IHC
Gene Id: 596
Isotype: Rabbit IgG
Format Id: Purified
Clone Name: BCL2/1878R
Uniprot Id: P10415
Host Animal: Rabbit
Clonality Id: Monoclonal
Purification: Protein A/G
Reactivity Id: Human
Product Content: 200µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Alternative Name: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
Application Note: Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),
Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information: Recombinant full-length human bcl-2 protein