Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability. This Medium provides superior quality, consistency, and great control in mammalian cell culture. DMEM F-12 is preferred for MDCK, glial cells, fibroblasts, human endothelial cells, as well as rat fibroblasts. DMEM-F12 is available in Standard, MAX ( contains L-Alanyl-L-glutamine), and Advanced ( Needs only 2-5% FBS) formulations.  Furthermore, PurMa Biologics currently manufactures 132 conditions of this media.