DESCRIPTION

• Notes: Troubleshooting Guide: Visit Assay-kits-faq for detailed troubleshooting instructions. For further questions, please email support@bpsbioscience.com.
• Uniprot: P04070
• Shiptemp: -80°C (dry ice)
• Synonyms: PROC, APC, activated protein C, active coagulation factor XIV
• Warnings: Avoid freeze/thaw cycles
• Category: Proteases/Assay Kit
• Background: Protein C, also known as autoprothrombin IIA and blood coagulation factor XIX, is a serine protease which acts as an anticoagulant and is normally expressed in its zymogen (pro-enzyme) form. Activated protein C (APC or PROC) inhibits coagulation by inactivating the proteolysis of Factor Va and Factor VIIIa and regulating blood vessel permeability and inflammatory responses. PROC activation is vitamin-K dependent and vitamin K deficiency or alterations in PROC activation can lead to increased risk of blood clot-related pathologies such as thrombosis.
• Description: The Activated Protein C (APC, also known as PROC) Assay Kit is a 96-well format fluorogenic assay designed to measure the protease activity of Activated Protein C (PROC) for screening and profiling applications. Illustration of the assay principle. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent AMC. Fluorescence intensity increases proportionally to the activity of the protease.  
• Supplied As: The kit contains sufficient amounts of purified recombinant PROC protein, PROC fluorogenic substrate, and PROC assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
• Scientific Category: Proteases