DESCRIPTION

• Mw: 55 kDa
• Tag: Myc-DDK
• Accn: NM_001199922
• Type: Human Tagged ORF Clone
• Refseq: NM_001199922.1, NP_001186851.1
• Status: 3 Weeks*
• Symbol: SIAE
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 54414
• Orf Size: 1464 bp
• Synonyms: AIS6; CSE-C; CSEC; LSE; YSG2
• Refseq Orf: 1467 bp
• Uniprot Id: Q9HAT2
• Refseq Size: 3038 bp
• Availability: 3 Weeks
• Cytogenetics: 11q24.2
• Gene Summary: This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene.[provided by RefSeq, Feb 2011]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC232838 representing NM_001199922 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGTGCTGCAGAAGGAGCCTGCTGGGGCAGTGATATGGGGCTTCGGTACACCTGGAGCCACAGTGACCG TGACCCTGCGCCAAGGTCAGGAAACCATCATGAAGAAAGTGACCAGTGTGAAAGCTCACTCTGATACGTG GATGGTGGTACTGGATCCTATGAAGCCTGGAGGACCTTTCGAAGTGATGGCACAACAGACTTTGGAGAAA ATAAACTTCACCCTGAGAGTTCATGACGTCCTGTTTGGAGATGTCTGGCTCTGTAGTGGGCAGAGTAACA TGCAGATGACTGTGTTACAGATATTTAATGCTACAAGGGAGTTGTCTAACACTGCGGCATATCAGTCTGT CCGCATCCTCTCTGTCTCTCCCATTCAAGCAGAGCAGGAGCTGGAGGACCTTGTTGCGGTTGACTTGCAG TGGTCTAAGCCCACCTCAGAAAACTTAGGCCATGGATATTTCAAGTACATGTCAGCAGTGTGCTGGCTCT TTGGACGTCACCTTTATGACACTCTGCAGTATCCCATCGGGCTGATCGCCTCCAGCTGGGGCGGGACACC CATTGAAGCCTGGTCATCTGGACGGTCACTGAAAGCCTGTGGGGTCCCTAAACAAGGGTCCATTCCATAC GATTCTGTAACTGGTCCCAGTAAGCACTCTGTTCTCTGGAATGCCATGATCCATCCACTGTGCAATATGA CTCTGAAAGGGGTAGTATGGTACCAGGGGGAGTCCAATATAAATTATAACACGGATCTGTACAATTGCAC ATTCCCTGCACTCATCGAAGACTGGCGTGAAACCTTCCACCGTGGTTCCCAGGGGCAGACGGAGCGTTTC TTCCCATTTGGACTTGTCCAGTTATCTTCAGATTTGTCTAAGAAGAGCTCAGACGATGGATTTCCCCAGA TCCGTTGGCATCAAACAGCAGACTTCGGCTATGTCCCCAACCCAAAGATGCCCAATACTTTCATGGCTGT AGCTATGGATCTCTGTGATAGAGACTCGCCTTTTGGCAGCATCCACCCTCGAGATAAACAGACTGTGGCT TATCGGCTGCATTTGGGGGCCCGTGCTCTGGCTTATGGTGAGAAGAATTTGACCTTTGAAGGACCACTGC CTGAGAAGATAGAACTCTTGGCTCACAAGGGGCTGCTCAATCTCACATATTACCAGCAAATCCAGGTGCA GAAAAAGGACAACAAGATATTTGAGATCTCCTGTTGCAGTGACCATCGATGCAAGTGGCTTCCAGCTTCT ATGAACACCGTCTCCACCCAGTCCCTGACCCTGGCGATCGATTCTTGTCATGGCACTGTGGTTGCTCTCC GCTATGCTTGGACCACGTGGCCTTGTGAATATAAGCAGTGTCCCCTATACCACCCCAGTAGTGCCCTGCC AGCCCCTCCCTTCATTGCTTTCATTACAGACCAGGGTCCTGGACATCAGAGCAATGTTGCTAAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC232838 representing NM_001199922 Red =Cloning site Green =Tags(s) MVLQKEPAGAVIWGFGTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQQTLEK INFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQSVRILSVSPIQAEQELEDLVAVDLQ WSKPTSENLGHGYFKYMSAVCWLFGRHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPY DSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDWRETFHRGSQGQTERF FPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYVPNPKMPNTFMAVAMDLCDRDSPFGSIHPRDKQTVA YRLHLGARALAYGEKNLTFEGPLPEKIELLAHKGLLNLTYYQQIQVQKKDNKIFEISCCSDHRCKWLPAS MNTVSTQSLTLAIDSCHGTVVALRYAWTTWPCEYKQCPLYHPSSALPAPPFIAFITDQGPGHQSNVAK TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin