DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: SYT1
• Host: Rabbit
• Rrid: AB_2492253
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: Synaptotagmin (Ser309)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Applications: WB, ICC
• Ncbi Gene Id: 25716
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 60/62
• Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:1000
• Immunogen Species: Rat
• Dilution Range Icc: 1:400
• Species Reactivity: Rat
• Target Description: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
• Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate, Mouse, Zebrafish
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P21707
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Ser309

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