DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: FMS, CD115
• Warnings: Avoid freeze/thaw cycles.
• Category: Cell Based Assay Kit
• Background: Colony Stimulating Factor 1 Receptor (CSF1R, CD115, M-CSF-R) is the receptor for the cytokine CSF1 (MCSF; CSF-1), which controls the growth, function, and differentiation of macrophages. This interaction drives the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer. An alternative ligand is IL-34, which has been implicated in neuroprotective and survival signals following brain injury and neurodegeneration. As a tyrosine kinase transmembrane receptor, activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
• References: 1. Cannarile, Michael A., et al. "Colony-Stimulating Factor 1 Receptor (CSF1R) Inhibitors in Cancer Therapy." J. Immunother. Cancer, 5(1): 53 (2017) 2. Liu, Yang, and Xuetao Cao. "The Origin and Function of Tumor-Associated Macrophages." Cellular Molec. Immunology, 12(1): 1-4 (2014). 3. Luo, Jian, et al. "Colony-Stimulating Factor 1 Receptor (CSF1R) Signaling in Injured Neurons Facilitates Protection and Survival." J. Exp. Med., 210(1): 157-172 (2013). 4. Yao, G.-Q., et al. "CSF-1 Induces Fos Gene Transcription and Activates the Transcription Factor Elk-1 in Mature Osteoclasts." Calc. Tissue Int., 76(5): 371-378 (2005).
• Description: The CSF1R SRE Reporter Kit is designed for monitoring the activity of the CSF1R signaling pathway in cultured cells. The kit contains a transfection-ready vector for CSF1R and SRE luciferase reporter vector. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
• Supplied As: Each kit contains sufficient reagents for 500 assays using a 96-well plate.
• Unspsc Code: 41106513
• Unspsc Name: Reporter gene assay
• Applications: • Monitor CSF1R/MAPK/ERK signaling pathway activity and SRF-mediated activity. • Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway. • Study effects of RNAi or gene overexpression.
• Product Type: Reporter Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 40227, 79110, 72550, 79343, 79385, 60690
• Storage Stability: Store cell line in liquid nitrogen immediately upon receipt. Store other components as described below. Stable for at least 6 months from date of receipt when stored as directed.
• Scientific Category: Immunotherapy
• Instructions for Use: See assay kit datasheet for detailed protocol.