DESCRIPTION

• Aa: 29-471
• Mw: 44.2 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_007371
• Species: Human
• Uniprot: Q15059
• Shiptemp: -80°C (dry ice)
• Synonyms: Bromodomain containing 3, BRD3, RING3L
• Warnings: Avoid freeze/thaw cycles.
• Category: Bromodomain/Protein
• References: Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
• Description: Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 - 417 corresponding to bromodomains 1 and 2, with N-terminal His-tag, MW = 44.2 kDa, expressed in an E. coli expression system
• Formulation: 40 mM HEPES, pH 7.2, 290 mM NaCl, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
• Host Species: E. coli
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® Ni acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Bromodomain