ESF AF™ Insect Cell Culture Medium is an Animal Origin-Free formulation and is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni, and Drosophila S2. ESF AF medium is comparable to ESF 921 for growth and expression. ESF AF contains L-Glutamine and Kolliphor® P188 (Pluronic® F-68). ESF AF is the logical choice for further manufacturing under cGMP/FDA regulatory compliance and has a Drug Master File with the US FDA.