DESCRIPTION

• Notes: The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
• Format: assay kit
• Shiptemp: -20°C
• Synonyms: SRE reporter, MAPK reporter, ERK reporter
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Cell Based Assay Kit/Cell Signaling Pathway
• Background: The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
• References: 1. Wong, K.K. (2009) Recent developments in anti-cancer agents targeting the Ras/Raf/ MEK/ERK pathway. Recent Pat Anticancer Drug Discov. 4(1):28-35.2. Treisman, R. (1992) The serum response element. Trends Biochem Sci. 17(10): 423-426.
• Description: The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
• Supplied As: Reporter: SRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
• Unspsc Code: 41106513
• Unspsc Name: Reporter gene assay
• Applications: • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.• Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway.
• Product Type: Reporter Assay Kit
• Concentration: Both the Reporter and Negative Control Reporter are provided at 55 ng DNA/µl (500 µl each)
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 60683-1, 60683-2, 60683-3, 79271, 60690, 60683
• Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)
• Scientific Category: Cell Signaling Pathway
• Instructions for Use: See data sheet for detailed transfection and assay protocols.