Lysis-EZ G Non-denaturing Cell Lysis Buffer, 1X Strength Solution: The Non-denaturing Cell Lysis Buffer contains no-ion detergent, salt, EDTA. The Lysis buffer is able to lyse cells from adherent cultures, suspension cultures as well as animal tissues. The Lysis buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins. Protein extracted can be readily used in many applications, including reporter assays, protein assays, immunoassays and protein purification. The Lysis Buffer is supplied with protease and phosphatase inhibitors that maintain protein integrity and high biological activity. Depending on the required downstream applications, additional agents such as reducing agents, other protease inhibitors may be added into the The Lysis Buffer, 10ml is sufficient for10x107 cells or 10x100mg tissue sample.