SARS-CoV-2 D614G Pseudovirus Neutralization Kit, Luciferase Reporter

DESCRIPTION

The SARS-CoV-2 D614G Reporter Virus Particle (RVP) Neutralization Kit allows your lab to screen antibodies or serum for neutralization against SARS-CoV-2 D614G in BSL-2 conditions. The kit includes target cells (HEK-293T hACE2 stable cells), 2 mL SARS-CoV-2 D614G RVPs (pseudovirus), 100 μg neutralizing control antibody, 1 mL VSV-g RVPs (specificity control, positive control for infectivity). Customer provides own tissue culture plates, cell culture medium and Renilla luciferase substrate kit.

DETAILS

Type: Neutralization Assay Kit
Method: Pseudovirus Neutralization
Category: Pseudovirus
Lead Time: 7-10 days
Application: Neutralization Assay
Subcategory: Reporter Virus Particles
Antibody Tds: https://www.integralmolecular.com/wp-content/uploads/2022/10/Integral-Antibody-IM-COV2-G06-lot-01192022-B02-Technical-Data-Sheet.pdf
Cell Protocol: https://www.integralmolecular.com/wp-content/uploads/2021/06/Cell-passaging-and-maintenance-of-293T-hsACE2-cells-1.pdf
Strainvariant: SARS-CoV-2 D614G
Pseudovirus Tds: Please refer to the lot specific TDS, following this link: https://www.integralmolecular.com/sarscov2rvpdocuments/
Infectivity Protocol: https://www.integralmolecular.com/wp-content/uploads/2022/09/Infectivity-of-SARS-CoV-2-Luciferase-RVPs-2.pdf
Neutralization Protocol: https://www.integralmolecular.com/wp-content/uploads/2022/09/Neutralization-of-SARS-CoV-2-Renilla-luciferase-RVPs.pdf
Cell Storage Temperature: LN2 upon receipt
Antibody Storage Temperature: -80 C (+/- 10 C) upon receipt; 4C upon thawing
Pseudovirus Storage Temperature: -80 C (+/- 10 C) upon receipt