20-1004
Polyclonal antibody to APAF-1
Abeomics
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DESCRIPTION
Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway, being the central element of the multimeric apoptosome.The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death.
DETAILS
- Gene: APAF1
- App Id: IP,IHC,WB
- Antigen: APAF-1
- Gene Id: 317
- Isotype: Rabbit IgG
- Format Id: Sera
- Uniprot Id: O14727
- Host Animal: Rabbit
- Clonality Id: Polyclonal
- Reactivity Id: Rat,Mouse,Human
- Product Content: 50 µl sera
- Alternative Name: APAF1||KIAA0413
- Application Note: WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200
- Storage Condition: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
- Immunogen Information: A full-length recombinant protein of APAF-1 was used as immunogen for this antibody
Equivalent Items
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