RC210463
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ARHGAP18 (NM_033515) Human Tagged ORF Clone
Origene Technologies, Inc.
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DESCRIPTION:
ARHGAP18 (Myc-DDK-tagged)-Human Rho GTPase activating protein 18 (ARHGAP18)
DETAILS
- Mw: 75.4 kDa
- Tag: Myc-DDK
- Accn: NM_033515
- Type: Human Tagged ORF Clone
- Refseq: NM_033515.3
- Status: In Stock*
- Symbol: ARHGAP18
- Vector: pCMV6-Entry
- Domains: RhoGAP
- Category: cDNA Clones
- Locus Id: 93663
- Orf Size: 1989 bp
- Synonyms: bA307O14.2; MacGAP; SENEX
- Refseq Orf: 1992 bp
- Uniprot Id: Q8N392
- Refseq Size: 3503 bp
- Availability: In Stock
- Cytogenetics: 6q22.33
- Gene Summary: ARHGAP18 belongs to a family of Rho (see MIM 165390) GTPase-activating proteins that modulate cell signaling (Potkin et al., 2009 [PubMed 19065146]).[supplied by OMIM, Apr 2010]
- Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC210463 representing NM_033515 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAGCTGGCTCTCCAGTTCCCAGGGAGTGGTACTAACAGCCTACCACCCCAGCGGCAAGGACCAGACCG TCGGGAACAGCCATGCAAAGGCAGGGGAGGAAGCCACCTCGAGTCGCAGATATGGCCAGTACACTATGAA CCAGGAAAGCACCACCATCAAAGTTATGGAGAAGCCTCCATTTGATCGATCAATTTCCCAGGATTCTTTG GATGAACTATCTATGGAAGACTATTGGATAGAACTAGAAAACATCAAGAAATCTAGTGAAAACAGCCAAG AAGATCAAGAGGTGGTTGTTGTCAAAGAGCCTGATGAGGGAGAATTGGAAGAAGAGTGGCTTAAAGAGGC CGGTTTATCCAATCTCTTCGGAGAGTCTGCTGGAGATCCACAGGAAAGCATTGTGTTTTTATCAACATTG ACGCGGACCCAGGCAGCAGCAGTTCAGAAGCGAGTAGAGACGGTCTCCCAGACCTTGAGGAAAAAAAACA AACAGTACCAGATTCCTGACGTCAGAGACATATTTGCTCAACAGAGAGAATCAAAAGAAACAGCTCCAGG TGGCACTGAATCGCAGTCACTTAGAACAAATGAAAACAAATACCAAGGAAGAGATGACGAGGCATCTAAC CTTGTTGGTGAAGAGAAGCTGATCCCACCTGAGGAGACGCCTGCCCCTGAAACAGACATCAACCTGGAGG TATCATTTGCCGAGCAAGCACTCAATCAGAAAGAGAGCTCCAAGGAGAAAATCCAGAAGAGCAAAGGCGA TGATGCCACATTACCTAGTTTCAGATTGCCAAAAGACAAAACGGGTACCACAAGGATTGGTGACCTCGCA CCCCAGGACATGAAGAAAGTTTGCCATTTAGCCCTAATTGAGCTGACTGCCCTCTATGATGTATTGGGTA TTGAGCTGAAACAACAAAAAGCTGTGAAAATCAAAACAAAAGATTCTGGTCTTTTTTGCGTTCCATTGAC AGCGCTATTAGAACAAGATCAGAGGAAAGTACCAGGAATGCGAATACCCTTGATCTTTCAAAAACTGATT TCTCGAATTGAAGAGAGAGGTTTGGAAACAGAAGGCCTCTTACGGATCCCTGGAGCTGCCATTAGAATCA AGAATCTTTGCCAAGAACTAGAAGCAAAGTTTTATGAAGGGACTTTTAATTGGGAAAGTGTCAAACAGCA TGATGCCGCCAGCCTGCTGAAGCTCTTCATTCGGGAGTTGCCCCAGCCACTGCTCAGTGTGGAGTATCTC AAAGCCTTTCAGGCTGTCCAGAATCTTCCAACCAAGAAGCAGCAACTACAGGCTTTGAACCTTCTTGTCA TCCTCCTACCTGATGCAAACAGGGACACACTGAAGGCCCTTCTTGAATTTCTCCAAAGAGTAATAGATAA TAAAGAAAAAAATAAAATGACAGTCATGAATGTAGCAATGGTCATGGCCCCGAATCTCTTTATGTGTCAT GCATTGGGATTGAAGTCCAGTGAACAGCGAGAATTTGTAATGGCAGCTGGGACAGCAAATACCATGCACT TATTGATTAAGTACCAAAAACTTCTGTGGACAATTCCCAAGTTTATTGTAAACCAAGTGAGGAAGCAAAA CACGGAAAATCATAAAAAGGATAAAAGAGCCATGAAGAAATTGCTGAAGAAAATGGCTTATGACCGAGAA AAATATGAAAAGCAAGATAAGAGTACAAATGATGCTGACGTTCCTCAGGGAGTGATTCGAGTGCAAGCTC CCCATCTTTCGAAAGTTTCCATGGCAATACAGCTAACTGAAGAACTAAAAGCCAGTGATGTACTTGCCAG GTTTCTCAGCCAAGAAAGTGGGGTTGCCCAGACTCTCAAGAAAGGAGAAGTTTTTTTGTATGAAATTGGA GGAAATATTGGGGAACGCTGCCTTGATGATGACACTTACATGAAGGATTTATATCAGCTTAACCCAAATG CTGAGTGGGTTATAAAGTCAAAGCCATTG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC210463 representing NM_033515 Red =Cloning site Green =Tags(s) MSWLSSSQGVVLTAYHPSGKDQTVGNSHAKAGEEATSSRRYGQYTMNQESTTIKVMEKPPFDRSISQDSL DELSMEDYWIELENIKKSSENSQEDQEVVVVKEPDEGELEEEWLKEAGLSNLFGESAGDPQESIVFLSTL TRTQAAAVQKRVETVSQTLRKKNKQYQIPDVRDIFAQQRESKETAPGGTESQSLRTNENKYQGRDDEASN LVGEEKLIPPEETPAPETDINLEVSFAEQALNQKESSKEKIQKSKGDDATLPSFRLPKDKTGTTRIGDLA PQDMKKVCHLALIELTALYDVLGIELKQQKAVKIKTKDSGLFCVPLTALLEQDQRKVPGMRIPLIFQKLI SRIEERGLETEGLLRIPGAAIRIKNLCQELEAKFYEGTFNWESVKQHDAASLLKLFIRELPQPLLSVEYL KAFQAVQNLPTKKQQLQALNLLVILLPDANRDTLKALLEFLQRVIDNKEKNKMTVMNVAMVMAPNLFMCH ALGLKSSEQREFVMAAGTANTMHLLIKYQKLLWTIPKFIVNQVRKQNTENHKKDKRAMKKLLKKMAYDRE KYEKQDKSTNDADVPQGVIRVQAPHLSKVSMAIQLTEELKASDVLARFLSQESGVAQTLKKGEVFLYEIG GNIGERCLDDDTYMKDLYQLNPNAEWVIKSKPL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Kanamycin (25 ug/mL)
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin