50223
BCL-XL TR-FRET Assay Kit
BPS Bioscience
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DESCRIPTION
The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL
binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay
requires no time-consuming washing steps, making it especially suitable for high throughput
screening applications. The assay procedure is straightforward and simple; a sample containing
terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is
incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence
reader.
DETAILS
- Shiptemp: -80°C (dry ice)
- Synonyms: Bcl-Xlong, B cell lymphoma XL
- Warnings: Avoid freeze/thaw cycles.
- Category: Apoptosis/Assay Kit
- References: Lessene, G., et al., Nature Chem. Biol. 2013 ; 9:390
- Description: The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
- Methodology: TR-FRET
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 50273, 50222
- Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: Apoptosis
- Instructions for Use: See assay kit datasheet for detailed protocol.