Anti-SMAD7 (N-terminal region) Antibody

DETAILS

Form: Protein G Purified
Gene: SMAD7
Host: Rabbit
Rrid: AB_3068506
Type: Primary Antibody
Buffer: PBS + 0.05% BSA and 0.05% NaN3
Target: SMAD7 (N-terminal region)
Isotype: IgG
Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Shipping: Blue Ice
Synonyms: MADH7, MADH8, MAD7
Clonality: Rabbit Polyclonal
Immunogen: This antibody was raised against amino acids from the N-terminal region of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 46
Dilution Range Wb: 1:500
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Human, Mouse, Rat, and Xenopus
Target Description: SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.
Uniprot Number Immunogen Species: O15105