Wnt10b Antibody

DETAILS

Buffer: Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
Isotype: IgG
Storage: Wnt10b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Category: Antibody
Shipping: Blue Ice
Clonality: Polyclonal
Conjugate: Unconjugated
Immunogen: Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b.The immunogen is located within amino acids 130 - 180 of Wnt10b.
Swissprot: O00744
User Note: Optimal dilutions for each application to be determined by the researcher.
Background: Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
Specificity: Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
Host Species: Rabbit
Ncbi Gene Id: 7480
Purification: Wnt10b Antibody is affinity chromatography purified via peptide column.
Concentration: 1 mg/mL
Modifications: None
Ncbi Organism: Homo sapiens
Physical State: Liquid
Additional Names: Wnt10b Antibody: SHFM6, WNT-12, WNT12, Protein Wnt-10b, Protein Wnt-12
Application Note: Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
By Research Area: Signal Transduction
Protein Accession: NP_003385
Protein Gi Number: 16936522
Ncbi Official Name: wingless-type MMTV integration site family, member 10B
Positive Control 1: Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity: Human,Mouse,Rat
Tested Applications: ELISA,WB,IHC-P
Ncbi Official Symbol: WNT10B
Predicted Molecular Weight: Predicted: 43 kDa Observed: 47 kDa