DESCRIPTION

• Mw: 70.8 kDa
• Tag: Myc-DDK
• Accn: NM_001005244
• Type: Rat Tagged ORF Clone
• Refseq: NM_001005244.2, NP_001005244.1
• Status: 5 Weeks*
• Symbol: Dmd
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 24907
• Orf Size: 1866 bp
• Synonyms: DNADMD1
• Refseq Orf: 1869 bp
• Refseq Size: 1990 bp
• Availability: 5 Weeks
• Cytogenetics: Xq21
• Gene Summary: a vital component of a muscle sarcolemma membrane-spanning complex that connects cytoskeleton to basal lamina; plays a role in retinal neurotransmission; mutations cause Duchenne muscular dystrophy [RGD, Feb 2006]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RR200462 representing NM_001005244 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAGGGAACACCTCAAAGGCCACGAGACCCAAACAACTTGTTGGGACCATCCCAAAATGACAGAGCTCT ACCAGTCTTTAGCTGACCTGAATAATGTCAGGTTCTCCGCTTATAGGACTGCCATGAAGCTCAGAAGACT CCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGACGCCTTGGACCAGCACAACCTC AAGCAAAATGATCAGCCCATGGATATCCTGCAGATAATTAACTGTTTGACTACTATTTATGACCGTCTGG AGCAAGAGCACAACAATTTGGTCAATGTCCCTCTCTGTGTGGATATGTGTCTCAACTGGCTTCTCAATGT TTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCTCTGTGTAAA GCACACTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACCGGCTTTTGTGACCAGC GTAGACTGGGTCTTCTTCTGCATGATTCTATTCAAATCCCAAGACAGTTGGGTGAAGTTGCCTCCTTTGG GGGCAGTAACATTGAGCCGAGTGTCAGGAGCTGTTTCCAGTTTGCCAATAATAAACCTGAGATTGAAGCC GCTCTCTTCCTTGACTGGATGCGTCTGGAACCCCAGTCTATGGTGTGGCTGCCGGTCTTGCACAGAGTGG CTGCTGCTGAAACAGCCAAGCATCAGGCCAAGTGTAACATCTGTAAGGAGTGTCCAATCATTGGATTCAG GTACAGAAGCCTGAAACACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGCCGAGTTGCTAAG GGCCATAAAATGCACTACCCCATGGTAGAGTACTGCACTCCGACTACATCTGGAGAAGATGTTCGTGACT TTGCCAAGGTACTGAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCT TCCAGTGCAGACTGTGTTAGAGGGGGACAACATGGAAACGCCTGCCTCGTCCCCCCAGCTTTCACACGAT GATACTCATTCACGCATTGAGCATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATC TAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAG TTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAG GAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGAAATCTGCAAGCAGAATATG ATCGTCTGAAGCAGCAGCATGAGCATAAAGGCCTGTCCCCACTGCCGTCTCCTCCTGAGATGATGCCCAC CTCTCCCCAGAGTCCCAGGGATGCTGAACTCATTGCTGAGGCCAAGCTACTGCGCCAACACAAAGGACGT CTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAACTGGAGTCTCAGTTACATAGACTGAGGC AGCTCCTGGAGCAACCCCAGGCTGAGGCCAAGGTGAATGGCACCACGGTGTCCTCTCCTTCTACCTCGCT GCAGAGGTCAGATAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCAGAATCTATGGGT GAGGAAGATCTTCTGAGTCCTCCCCAGGACACAAGCACAGGGTTAGAAGAAGTGATGGAGCAACTCAACA ACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGCCTTTTCCACATGGCAGATGATTTGGGCAGAGC GATGGAGTCCTTAGTTTCAGTCATGATAGATGAAGAAGGAGCAGAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RR200462 representing NM_001005244 Red =Cloning site Green =Tags(s) MREHLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNL KQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCK AHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEA ALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAK GHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHD DTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESE ERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGR LEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMG EEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGHNVGSLFHMADDLGRAMESLVSVMIDEEGAE TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin