79936
ACE2: Spike S1 RBD (SARS-CoV-2) Inhibitor Screening Assay Kit
BPS Bioscience
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DESCRIPTION
The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of bound Fc-tagged Spike protein by HRP-labeled Anti-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with Anti-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
DETAILS
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles.
- Category: Coronavirus/Assay Kit
- Background: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.
- References: Hoffmann, M. et al. 2020. Cell, 181:1-10Yan, R. et al. Science, In Press 4 March 2020. DOI: 10.1126/science.abb2762
- Description: The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of bound Fc-tagged Spike protein by HRP-labeled Anti-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with Anti-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: This kit comes in a convenient 96-well format, with ACE2, purified SARS-CoV-2 Spike Fc fusion protein, HRP-labeled anti-Fc antibody, and assay buffer for 100 binding reactions.
- Unspsc Code: 41105332
- Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
- Applications: This kit is useful for screening inhibitors of ACE2 binding to SARS-CoV-2 Spike.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 79670, 11003, 79923, 79931
- Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
- Scientific Category: Infectious Disease
- Instructions for Use: See data sheet for detailed instructions.