DESCRIPTION

• Shiptemp: +4°C (gel packs)
• Synonyms: Cell lysis buffer
• Category: Cell Signaling/Buffer
• Description: The Modified RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables the extraction of cytoplasmic and nuclear proteins in mild denaturing conditions. This is an SDS-free buffer optimized and formulated for proteins that might be sensitive to some components of the standard RIPA Buffer. This buffer is compatible with many applications, including ELISA-based assays.
• Formulation: 50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% Triton X-100, and 0.5% sodium deoxycholate
• Unspsc Code: 47101613
• Unspsc Name: Buffer solutions
• Product Type: Buffers and Biochemical Reagents
• Biosafety Level: Not applicable (BSL-1)
• Storage Stability: Upon receipt, store at 4°C.  Stable for 6 months from date of receipt, when stored as directed.
• Mycoplasma Testing: Sterile filtered (0.2 µm)
• Scientific Category: Consumable
• Instructions for Use: This is a ready-to-use buffer. Mix well before use. This buffer does not contain protease or phosphatase inhibitors. If desired, add appropriate inhibitors (such as protease inhibitors, phosphatase inhibitors, ADP-ribosylation inhibitors etc.) immediately before use to prevent protein proteolysis or to maintain phosphorylation and/or ribosylation status of proteins.
• Signed License Required: No