ACC-002_25 mcl ACC-002_50 mcl ACC-002_0.2 ml ACC-002-CF_0.2 ml
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Anti-CACNA1B (CaV2.2) Antibody
Alomone Labs
DESCRIPTION
Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.2 from mouse, rat, and human samples.
DETAILS
- Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
- Host: Rabbit
- Clone: NA
- Label: Unconjugated
- Purity: Affinity purified on immobilized antigen.
- Target: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 257648
- Isotype: Rabbit IgG
- Homology: Mouse - 15/17 amino acid residues identical; human 11/17 amino acid residues identical; rabbit - 11/17 amino acid residues identical
- Is Toxin: No
- Sequence: (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B
- Synonyms: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII
- Accession: Q02294
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4
- Applications: IC|IF|IHC|IP|WB
- Ko-validated: yes
- Preservative: No Preservative
- Reconstitution: 0.2 ml double distilled water (DDW).
- Blocking Peptide: BLP-CC002
- Negative Control: BLP-CC002
- Positive Control: NA
- Cited Application: IP|IHC
- Immunogen Location: Intracellular loop between domains II and III
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Rat
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: IC|IF|IHC|IP|WB
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page - Scientific Background: Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N-type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4 ω-Conotoxin GVIA (#C-300) is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.5,6
- Standard Quality Control of Each Lot: Western blot analysis
- Application Dilutions Western Blot Wb: 1:200
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:100