Anti-Fibronectin Antibody

DETAILS

Form: Protein G Purified
Gene: FN1
Host: Mouse
Rrid: AB_3068442
Type: Primary Antibody
Clone: M022
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Fibronectin
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1
Clonality: Mouse Monoclonal
Immunogen: Clone (M022) was generated from a proprietary antigen related to native human fibronectin from the MeWo melanoma cell line.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: Clone M022 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of native or denatured human A549, MeWo, and LNCaP cells, as well as human plasma fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 250
Dilution Range Ip: 1:100
Dilution Range Wb: 1:500
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.
Uniprot Number Immunogen Species: P02751
Post Translational Modification Type: GOmAb