DESCRIPTION

• Mw: 79.3 kDa
• Tag: Myc-DDK
• Accn: NM_001136034
• Type: Human Tagged ORF Clone
• Refseq: NM_001136034.1, NP_001129506.1
• Status: In Stock*
• Symbol: DDX4
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 54514
• Orf Size: 2172 bp
• Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; OTTHUMP00000122546; VASA
• Refseq Orf: 2174 bp
• Refseq Size: 2880 bp
• Availability: In Stock
• Cytogenetics: 5q11.2
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC226957 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAGG ATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGATGG ACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGATGCT GGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGAAACA GAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAATGACTG CGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGGCTATCGAGATGGAAATAATTCAGAA GCTTCAGGGCCATACAGAAGAGGTGGAAGAGGTAGTTTCCGAGGTTGCCGTGGAGGATTTGGTCTAGGAA GTCCAAATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGGCCTTTTTGGTTCTAGAAGACC AGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGCAGTGGAAGTGAACGAGGTGGT TACAAAGGTTTAAATGAAGAAGTAATAACAGGCTCTGGAAAGAATTCTTGGAAGTCAGAAGCAGAAGGAG GAGAAAGTAGTGATACTCAAGGACCAAAAGTGACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTC CATCTTTGCACATTATCAGACAGGCATAAACTTCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGA CATGATGCACCACCAGCAATTCTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTG CTAAAGCTGGTTATACTAAGCTTACTCCTGTGCAAAAATACAGTATTCCTATCATACTTGCAGGACGAGA TTTGATGGCTTGCGCTCAAACAGGGTCTGGGAAGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATG ATGCATGATGGAATAACTGCCAGTCGTTTTAAAGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAA CTCGAGAATTGGTCAACCAGATTTATTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGT TGTTATATATGGGGGAACCCAGCTGGGACATTCAATTCGACAAATAGTACAAGGCTGTAATATATTATGT GCTACTCCTGGAAGACTGATGGATATCATAGGCAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAG TTTTGGATGAAGCTGATCGCATGTTGGATATGGGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCC AGGAATGCCATCAAAGGAACAGCGCCAAACCCTTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGG TTGGCTGCAGAGTTTTTAAAGTCAAATTATCTGTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAG ATGTTCAGCAGACCGTTCTCCAAGTTGGCCAGTTCTCAAAAAGAGAAAAGCTCGTTGAAATTCTGCGAAA CATAGGGGATGAAAGAACTATGGTCTTTGTTGAAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTT TGTCAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTG GAGATTTTCGCTTTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGA AAATGTGCAACATGTTATCAATTTTGATCTTCCTTCTACCATTGATGAATATGTTCATCGAATTGGGCGT ACTGGTCGTTGTGGGAATACTGGCAGAGCAATTTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCAC AGCCTCTAGTAAAAGTATTGACAGATGCTCAACAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAG TACATACATTCCTGGCTTCAGTGGTAGTACAAGAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGC AAGAGCACTTTGAACACAGCTGGGTTTTCTTCTTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGG AT ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC226957 protein sequence Red =Cloning site Green =Tags(s) MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDA GECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSE ASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGG YKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSG HDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHM MHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILC ATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQR LAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFL CQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGR TGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKG KSTLNTAGFSSSQAPNPVDDESWD TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin