SEK10143
Human CHL-1 ELISA Pair Set
Sino Biological
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DESCRIPTION
The Human CHL-1 ELISA Pair Set is for the quantitative determination of Human CHL-1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CHL-1 coated on a 96-well plate. Standards and samples are added to the wells, and any CHL-1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CHL-1 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CHL-1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 80 ng of recombinant CHL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 31.25-2000
- Specificity: Human CHL-1
- Capture Antibody: 0.2 mg/mL of mouse anti-CHL1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10143-MM05)
- Detection Antibody: 0.5 mg/mL mouse anti-CHL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10143-MM01)