SEK10936
Human GDF15 ELISA Pair Set
Sino Biological
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DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 15 ng of recombinant Human GDF15. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 4.69-300
- Specificity: Human GDF15
- Capture Antibody: 1 mg/mL of mouse anti-Human GDF15 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10936-MM09)
- Detection Antibody: 0.2 mg/mL of mouse anti-Human GDF15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.15 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10936-MM12)
DESCRIPTION
The Human GDF15 ELISA Pair Set is for the quantitative determination of Human GDF15.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human GDF15 coated on a 96-well plate. Standards and samples are added to the wells, and any Human GDF15 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human GDF15 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human GDF15 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.